4 posts · 1,003 views
An engineering perspective on circulating tumor cell isolation technology and down-stream analyses enabled by these techniques.
Erica Pratt
4 posts
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- December 10, 2012
- 09:00 AM
- 137 views
CTC genetic heterogeneity, a window into the metastatic process
by pratt_ed in CTC Engineer
Tumor genetic heterogeneity has emerged as an effective biomarker of malignant processes1-4. However, limited access to tissue in solid tumors makes repeated sampling and tracking of tumor genetic evolution infeasible. CTCs can serve as a “liquid biopsy”, allowing researchers to understand genetic heterogeneity of the cancer process in real time. The paper I’m reviewing today, “Single Cell Profiling of Circulating Tumor Cells: Transcriptional Heterogeneity and Diversity from Breast Cance........ Read more »
Powell Ashley A, Talasaz Amirali H, Zhang Haiyu, Coram Marc A, Reddy Anupama, Deng Glenn, Telli Melinda L, Advani Ranjana H, Carlson Robert W, & Mollick Joseph A. (2012) Single cell profiling of circulating tumor cells: transcriptional heterogeneity and diversity from breast cancer cell lines. PloS one. PMID: 22586443
- December 4, 2012
- 09:00 AM
- 184 views
Evaluating CTC isolation device performance
by pratt_ed in CTC Engineer
I’ve previously discussed how to sort CTCs, and the standards used to characterize device performance. Today, I’ll explain what some of the most common evaluation metrics are, and place them in context of eventual clinical/industrial application.... Read more »
Marrinucci Dena, Bethel Kelly, Lazar Daniel, Fisher Jennifer, Huynh Edward, Clark Peter, Bruce Richard, Nieva Jorge, & Kuhn Peter. (2010) Cytomorphology of circulating colorectal tumor cells:a small case series. Journal of oncology. PMID: 20111743
Kirby Brian J, Jodari Mona, Loftus Matthew S, Gakhar Gunjan, Pratt Erica D, Chanel-Vos Chantal, Gleghorn Jason P, Santana Steven M, Liu He, & Smith James P. (2012) Functional characterization of circulating tumor cells with a prostate-cancer-specific microfluidic device. PloS one. PMID: 22558290
- November 30, 2012
- 09:22 AM
- 499 views
Cancer Cell Lines & CTCs: Benchmarking versus Application
by pratt_ed in CTC Engineer
In my “How to Sort CTCs” series, I covered a variety of sorting methodologies used for patient prognosis. However, before clinical implementation, it is important characterize device performance with a series of standards. This is impossible to do with a patient blood sample, because there is an unknown number of CTCs floating around with other blood cells, which can be effected by the cancer treatment process (e.g. radiation patients often have anemia)1. Furthermore, this is all changing d........ Read more »
Kirby Brian J, Jodari Mona, Loftus Matthew S, Gakhar Gunjan, Pratt Erica D, Chanel-Vos Chantal, Gleghorn Jason P, Santana Steven M, Liu He, & Smith James P. (2012) Functional characterization of circulating tumor cells with a prostate-cancer-specific microfluidic device. PloS one. PMID: 22558290
Lazar Daniel C, Cho Edward H, Luttgen Madelyn S, Metzner Thomas J, Uson Maria Loressa, Torrey Melissa, Gross Mitchell E, & Kuhn Peter. (2012) Cytometric comparisons between circulating tumor cells from prostate cancer patients and the prostate-tumor-derived LNCaP cell line. Physical biology. PMID: 22306736
Powell Ashley A, Talasaz Amirali H, Zhang Haiyu, Coram Marc A, Reddy Anupama, Deng Glenn, Telli Melinda L, Advani Ranjana H, Carlson Robert W, & Mollick Joseph A. (2012) Single cell profiling of circulating tumor cells: transcriptional heterogeneity and diversity from breast cancer cell lines. PloS one. PMID: 22586443
Magbanua Mark Jesus M, Sosa Eduardo V, Roy Ritu, Eisenbud Lauren E, Scott Janet H, Olshen Adam, Pinkel Dan, Rugo Hope, & Park John W. (2012) Genomic profiling of isolated circulating tumor cells from metastatic breast cancer patients. Cancer research. PMID: 23135909
Borrell Brendan. (2010) How accurate are cancer cell lines?. Nature, 463(7283), 858-858. DOI: 10.1038/463858a
Hofman V. J., Ilie M. I., Bonnetaud C., Selva E., Long E., Molina T., Vignaud J. M., Flejou J. F., Lantuejoul S., & Piaton E. (2010) Cytopathologic Detection of Circulating Tumor Cells Using the Isolation by Size of Epithelial Tumor Cell Method: Promises and Pitfalls. American Journal of Clinical Pathology, 135(1), 146-156. DOI: 10.1309/AJCP9X8OZBEIQVVI
Stott Shannon L, Hsu Chia-Hsien, Tsukrov Dina I, Yu Min, Miyamoto David T, Waltman Belinda A, Rothenberg S Michael, Shah Ajay M, Smas Malgorzata E, & Korir George K. (2010) Isolation of circulating tumor cells using a microvortex-generating herringbone-chip. Proceedings of the National Academy of Sciences of the United States of America. PMID: 20930119
Cho Edward H, Wendel Marco, Luttgen Madelyn, Yoshioka Craig, Marrinucci Dena, Lazar Daniel, Schram Ethan, Nieva Jorge, Bazhenova Lyudmila, & Morgan Alison. (2012) Characterization of circulating tumor cell aggregates identified in patients with epithelial tumors. Physical biology. PMID: 22306705
Tormoen Garth W, Cianchetti Flor A, Bock Paul E, & McCarty Owen J T. (2012) Development of coagulation factor probes for the identification of procoagulant circulating tumor cells. Frontiers in oncology. PMID: 22973554
- November 26, 2012
- 10:25 AM
- 183 views
How to Sort Circulating Tumor Cells Part IV: Electrokinetic Separation
by pratt_ed in CTC Engineer
Most CTC sorting devices target some observed cancer cell phenotype that was determined from studying tumor tissue directly, or from using immortalized cancer cell lines. This means that active sorting techniques, like size-based selection and immunocapture, require some level of a priori knowledge about CTCs before you can engineer a device to capture them. Microscopic characterization is one CTC identification method that circumvents this problem, fixing (killing) the cells, and then using ima........ Read more »
Pratt Erica D., Huang Chao, Hawkins Benjamin G., Gleghorn Jason P., & Kirby Brian J. (2011) Rare cell capture in microfluidic devices. Chemical Engineering Science, 66(7), 1508-1522. DOI: 10.1016/j.ces.2010.09.012
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